M6250

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M6250

Aldrich

 

2-Mercaptoethanol

≥99.0%

Synonym:β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
CAS Number:60-24-2
Linear Formula:HSCH2CH2OH
Molecular Weight:78.13
Beilstein Registry Number:773648
EC Number:200-464-6
MDL number:MFCD00004890
PubChem Substance ID:24897085

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Description

ApplicationBME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
 Widely used for retarding oxidation of biological compounds in solution.
Packaging100, 250, 500 mL in glass btl

Properties

vapor density2.69 (vs air)
vapor pressure1 mmHg ( 20 °C)
assay≥99.0%
expl. lim.18 %
concentration14.3 M (pure liquid)
refractive indexn20/D 1.500(lit.)
bp157 °C(lit.)
density1.114 g/mL at 25 °C(lit.)

Safety

Personal Protective EquipmentFaceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
Hazard CodesT,N
Risk Statements20/22-24-34-51/53
Safety Statements26-36/37/39-45-61
RIDADRUN 2966 6.1/PG 2
WGK Germany3
RTECSKL5600000
UEL18.00%
LEL2.30%
Flash Point(F)171 °F
Flash Point(C)77 °C

References

MerckMerck 13,5894
BeilsteinBeil. 1,IV,2428
referenceAldrich MSDS 1, 1162:C / Corp MSDS 1 (2), 2209:A / FT-IR 2 (1), 415:B / FT-IR 1 (1), 262:B / FT-NMR 1 (1), 422:B / IR-Spectra (2), 138:E / IR-Spectra (3), 154:F / NMR-Reference 2 (1), 228:B / RegBook 1 (1), 281:L / Sax 6, 1744 / Sigma FT-IR 1 (2), 700:C / Structure Index 1, 38:D:7 / Vapor Phase 3, 344:C